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Explain The Process Of Recombinant Dna Technology
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Recombinant DNA is a molecule of DNA that has been modified to include genes from multiple sources either through genetic recombination or through laboratory techniques In the lab bacteria can be transformed with recombinant DNA Genetic recombination occurs during meiosis in a process known as crossing over
The possibility for recombinant DNA technology emerged with the discovery of restriction enzymes in 1968 by Swiss microbiologist Werner Arber The following year American microbiologist Hamilton O Smith purified so called type II restriction enzymes which were found to be essential to genetic engineering for their
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Process Of DNA Recombinant Technology Applications Steps Embibe
Process Of DNA Recombinant Technology Applications Steps Embibe
Using recombinant DNA technology and synthetic DNA any DNA sequence can be created and introduced into living organisms Molecular cloning is the laboratory process used to produce recombinant DNA It is one of two most widely used methods along with polymerase chain reaction PCR used to direct the replication of any specific DNA
Recombinant DNA technology is an extremely important research tool in biology It allows scientists to manipulate DNA fragments in order to study them in the lab It involves using a variety of laboratory methods to put a piece of DNA into a bacterial or yeast cell Once in the bacteria or yeast will copy the DNA along with its own
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Recombinant DNA Technology
Recombinant DNA Technology
Like so many genetic technologies used today recombinant DNA technology had its origins in the late 1960s and early 1970s By the 1960s scientists had already learned that cells repair DNA
Recombinant rDNA technology involves procedures for analyzing or combining DNA fragments from one or several organisms Figure 1 including the introduction of the rDNA molecule into a cell for its replication or integration into the genome of the target cell Figure 1 Recombinant DNA is made from combining DNA from different sources
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The possibility for recombinant DNA technology emerged with the discovery of restriction enzymes in 1968 by Swiss microbiologist Werner Arber The following year American microbiologist Hamilton O Smith purified so called type II restriction enzymes which were found to be essential to genetic engineering for their
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4 Ligation of DNA Molecules The purified DNA and the vector of interest are cut with the same restriction enzyme This gives us the cut fragment of DNA and the cut vector that is now open The process of joining these two pieces together using the enzyme DNA ligase is ligation The resulting DNA is recombinant DNA
The possibility for recombinant DNA technology emerged with the discovery of restriction enzymes in 1968 by Swiss microbiologist Werner Arber The following year American microbiologist Hamilton O Smith purified so called type II restriction enzymes which were found to be essential to genetic engineering for their
4 Ligation of DNA Molecules The purified DNA and the vector of interest are cut with the same restriction enzyme This gives us the cut fragment of DNA and the cut vector that is now open The process of joining these two pieces together using the enzyme DNA ligase is ligation The resulting DNA is recombinant DNA
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